Difference between Colloids and Crystalloids for DMLT and Paramedical students

 Difference between Colloids and Crystalloids 

NOTES :

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Electrochemistry topic in kannada medium for Paramedical and DMLT students

Electrochemistry

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Classification of Bacteria in Kannada medium For DMLT DOTAT DOTT DDT students

Bacteria :

Classification of Bacteria : 

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Aqua Regia In Kannada medium For DMLT and Paramedical students

Aqua Regia Topic in Kannada for Paramedical and DMLT students 

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X-RAY DEFINATION AND USES IN KANNADA MEDIUM FOR DMLT AND PARAMEDICAL STUDENTS

 X-RAY DEFINATION , PRODUCTION AND USES FOR PARAMEDICAL FIRST YEAR STUDENTS

VIDEO IN KANNADA EXPLAINATION :

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PROKARYOTIC AND EUKARYOTIC CELL IN KANNADA MEDIUM FOR DMLT AND PARAMEDICAL STUDENTS

 PROKARYOTIC AND EUKARYOTIC CELL IN KANNADA MEDIUM FOR DMLT AND PARAMEDICAL STUDENTS

BASIC BIOLOGY FOR FIRST YEAR PARAMEDICAL IMPORTANT TROPICS 

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PLASTER OF PARIS TOPIC IN KANNADA MEDIUM FOR DMLT AND PARAMEDICAL STUDENTS

 PLASTER OF PARIS TOPIC IN KANNADA MEDIUM FOR DMLT AND PARAMEDICAL STUDENTS

BASIC FIRST YEAR CHEMISTRY IMPORTANT TOPIC

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PASTER OF PARIS AND USE FOR DMLT AND PARAMEDICAL STUDENTS KARNATAKA

 PASTER OF PARIS AND USE FOR DMLT AND PARAMEDICAL 

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coordination compounds in kannada medium for dmlt and all paramedical students KARNATAKA

 DMLT AND PARAMEDICAL COURSE BASIC CHEMISTRY 

COORDINATION COMPOUNDS AND USES IN MEDICINE:

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DMLT AND DOTAT -2 YEAR STUDENTS MICROBIOLOGY NOTES

 DMLT AND DOTAT -2 YEAR STUDENTS 

IMPORTANT FOR EXAM POINT MICROBIOLOGY NOTES 

SCIENTISTS NAMES AND ROLES :

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PHOTOMETER-Transmittance,Absorbance for dmlt students

PHOTOMETER:

When light is passed through a coloured solution, certain wavelengths are selectively absorbed giving a plot of the absorption spectrum of the compound in solution. The wavelength at which maximum absorption is called the absorption  maximum (λ) of that compound. The light that is not absorbed is transmitted through the solution and gives the solution its colour.

Photometric instruments measure transmittance, which is defined as follows:

                                      Intensity of the emergent (or transmitted) light           Ie

Transmittance (T)= ------------------------------------------------------------- = ------

                                                  Intensity of the incident light                                 Io

Transmittance is usually expressed on a range of 0 to 100%.

If the concentration of the substance in solution is increased linearly, or if the path length that the light beam has to traverse is increased, transmittance falls exponentially. So a term absorbance is defined so that it is directly proportional to the concentration of the substance.

                                                           Ie

Absorbance (A)= log1/T = log  ----

                                                           Io

Absorbance has no units. Photometric instruments electronically convert the measured transmittance to absorbance values.

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COLORIMETER – PRINCIPLE, COMPONENTS, WORKING & APPLICATIONS

COLORIMETER – PRINCIPLE, COMPONENTS, WORKING & APPLICATIONS

PRINCIPLE OF COLORIMETER:

⇒ Colorimeter is based on the photometric technique which states that When a beam of incident light of intensity I₀ passes through a solution, a part of the incident light is reflected (I_r), a part is absorbed (I_a) and rest of the light is transmitted (I_t)

The mathematical relationship between the amount of light absorbed and the concentration of the substance can be shown by the two fundamental laws of photometry on which the colorimeter is based.

Beer’s Law

⇒ This law states that the amount of light absorbed is directly proportional to the concentration of the solute in the solution.

Lambert’s Law

⇒ The Lambert’s law states that the amount of light absorbed is directly proportional to the length and thickness of the solution under analysis.

PARTS OF COLORIMETER:

There are 5 essential parts in a calorimeter.

⇒ Light Source – The most common source of light used in colorimeter is a tungsten filament.

⇒ Monochromator – To select the particular wavelength filter or monochromators are used to split the light from light source.

⇒ Sample holder – Test tube or Cuvettes are used to hold the color solutions they are made up of Glass at the visible wavelength.

⇒ Photo Detector System – when light falls on the detector system, an electric current is generated, this reflects the Galvanometer reading.

⇒ Measuring device – The current from the detector is fed to the measuring device, the Galvanometer, shows the meter reading that is directly proportional to the intensity of light.

WORKING OF THE COLORIMETER:

⇒ When using a colorimeter, it requires being calibrated first which is done by using the standard solutions of the known concentration of the solute that has to be determined in the test solution.

⇒There is a ray of light with a certain wavelength that is specific for the assay is directed towards the solution. Before reaching the solution the ray of light passes through a series of different filters and lenses.

These lenses are used for navigation of the colored light in the colorimeter and the filter splits the beam of light into different wavelength and allows the required wavelength to pass through it and reaches the cuvette containing the standard or Test solutions.

It analyzes the reflected light and compares with a predetermined standard solution.

⇒ The galvanometer measures the electrical signals and displays it in the digital form. That digital representation of the electrical signals is the absorbance or optical density of the solution analyzed.

⇒ If the absorption of the solution is higher than there will be more light absorbed by the solution and if the absorption of the solution is low then more lights will be transmitted through the solution which affects the galvanometer reading and corresponds to the concentration of the solute in the solution.

APPLICATIONS OF THE COLORIMETER:

⇒ The colorimeter is commonly used for the determination of the concentration of a colored compound by measuring the optical density or its absorbance.

⇒ It can also be used for the determination of the course of the reaction by measuring the rate of formation and disappearance of the light absorbing compound in the range of the visible spectrum of light.

⇒ By colorimeter, a compound can be identified by determining the absorption spectrum in the visible region of the light spectrum.

Simple staining Microbiology for dmlt students

Simple Staining Procedure and its Mechanism:

Simple staining is a method of staining in which bacteria are stained by using a single stain.

Simple staining is also called as monochrome staining or positive staining.

Examples of simple stain are Methylene blue, Safranin, Malachite green, Basic fuchsin and crystal violet etc.

In simple staining procedure cell are uniformly stained.

Procedure of simple staining:

A clean grease free slide is taken .

A grease free slide is is made by first washing the slide with detergent wiping the excess water and the slide is passed through flame.

On these grease free slide smear is made by using a sterile wireloop and cell suspension.

These slide is allowed to air dry .

After air drying these slide is rapidly passed through a flame for three to four times for heat fixation.

After heat fixation the slide is placed on the staining rack and flooded with a particular stain (Methylene blue, Safranin, Malachite green, Basic fuchsin and crystal violet ) and these stain is allowed to react for three minutes.

Futher the slide is washed under running water.

The slide is air dried and washed under oil immersion.

Application :

Simple staining procedure stain bacteria easily and helps in observation under microscope.

It is useful in preliminary studies of morphological characters of cell that is its size, shape and arrangement.